PAMPA (parallel artificial membrane permeability assay) and other membrane systems

In the PAMPA experiment a semipermeable membrane is placed between two compartments, the donor and the acceptor compartment. The donor compartment simulates the intestinal fluid, hence FaSSIF or FeSSIF. The acceptor compartment contains simulated blood buffer, which is commonly a phosphate buffer with a pH 7.4. The test compound is introduced into the donor compartment. The compound then diffuses through the membrane into the acceptor compartment where samples are taken and analysed. The compound concentration in the acceptor compartment is measured at different time points. The time-concentration profile indicates how quickly the compound permeates through the membrane. These results can then be translated to predict compound uptake into the body.


C. Markopoulos et al.: In Vitro and Ex Vivo Investigation of the Impact of Luminal Lipid Phases on Passive Permeability of Lipophilic Small Molecules Using PAMPA (2013)

Elenaz Naderkhani et al.: Biomimetic PVPA in vitro model for estimation of the intestinal drug permeability using fasted and fed state simulated intestinal fluids (2015)

Hanady Ajine Bibi et al.: PermeapadTM for investigation of passive drug permeability: The effect of surfactants, co-solvents and simulated intestinal fluids (FaSSIF and FeSSIF) (2015)