Test the solubility of your test compound in simulated stomach and intestinal fluids. You can evaluate the effect of natural ingredients (bile salts and lecithin) of the human stomach and intestine on the solubility of your compound. It will help you to find out the strengths and weaknesses of your test compound under different conditions of the human stomach and intestine during different stages of drug and formulation development.
Biorelevant solubility tests can be performed in different ways. You can assess equilibrium or dynamic solubility of your compound depending on the time you take the samples.
The following sections will demonstrate different ways of running a solubility test from the simple shake flask method to more sophisticated methods using the µDiss or the Covaris apparatus.