Solubility tests

Test the solubility of your test compound in simulated stomach and intestinal fluids. You can evaluate the effect of natural ingredients (bile salts and lecithin) of the human stomach and intestine on the solubility of your compound. It will help you to find out the strengths and weaknesses of your test compound under different conditions of the human stomach and intestine during different stages of drug and formulation development.

Biorelevant solubility tests can be performed in different ways. You can assess equilibrium or dynamic solubility of your compound depending on the time you take the samples.

The following sections will demonstrate different ways of running a solubility test from the simple shake flask method to more sophisticated methods using the µDiss or the Covaris apparatus.

Shake flask

The shake flask method is the basic way of performing a solubility test. It requires simple equipment such as lockable flasks (conical flask, laboratory bottle, falcon tube), an oscillating shaker, an oven or incubator, syringes and syringe filters.

Uniprep

The UniPrep solubility method is a simplified shake flask method and a very convenient way of assessing compound solubility. It only requires the UniPrep filters, an oscillating shaker and an oven or incubator for the test.

µDiss Profiler

The µDiss Profiler is a multifunctional apparatus, which can be used for solubility assessment. The advantage of using µDiss is that no additional equipment is required to perform the tests.

Covaris ultrasonicator

The Covaris apparatus can be used for small quantities of compound during early stage development. It also can reduce your experiment duration.