FeSSCoF: How to make

How much do you want to make?

FeSSCoF






L

To make <%= amount.decimal_rtrim(NUMERICIAL_PRECISION) %> L of <% if (media_type == DOGFASSIF) { %>Dog FaSSIF <% } else if (media_type == DOGFASSGF) { %> Dog FaSSGF <% } else { %> <%= media_name %><% } %> <% if (media_type == DOGFASSGF) { %> pH <%= ph %><% } %> you will need:

<% if (little_amount) { %>

We recommend making at least <%= amount_threshold %> L of <%= media_name %> for reasons of accuracy.


<% } %>
BIORELEVANT POWDER
<%= sif.decimal_rtrim(NUMERICIAL_PRECISION) %> g of <%= product_name %>
<% if(sugestion.length > 0){ %>

You will need: <% var sep =''; _.each(sugestion, function(item) { %><%= sep %><%= item['quantity'] %> x <%= item['product_code'] %><% sep = ', '; }) %>

Add to Cart
<% }else{ %> To buy this quantity of powder, please contact us: orders@biorelevant.com <% } %>
BUFFER
<% if (naoh) { %>
<%= naoh.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Sodium hydroxide pellets (NaOH)
<% } %> <% if (hydrate) { %>
<%= hydrate.decimal_rtrim(NUMERICIAL_PRECISION) %> g of <%= hydrate_name %>
<% } %> <% if (gaa) { %>
<%= gaa.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Glacial acetic acid
<% } %> <% if (maleic) { %>
<%= maleic.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Maleic acid (C4H4O4)
<% } %> <% if (nacl) { %>
<%= nacl.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Sodium chloride (NaCl)
<% } %> <% if (tris_base) { %>
<%= tris_base.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Tris base (C4H11NO3)
<% } %>
1

Prepare <% if (media_name === FaSSGF) { %> HCl/NaCl solution <% } else { %> buffer <% } %>

In about <%= h2o.decimal_rtrim(NUMERICIAL_PRECISION) %> L of purified water dissolve:

<% if (naoh) { %>
<%= naoh.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Sodium hydroxide pellets
<% } %> <% if (hydrate) { %>
<%= hydrate.decimal_rtrim(NUMERICIAL_PRECISION) %> g of <%= hydrate_name_c %>
<% } %> <% if (gaa) { %>
<%= gaa.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Glacial acetic acid
<% } %> <% if (maleic) { %>
<%= maleic.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Maleic acid
<% } %> <% if (nacl) { %>
<%= nacl.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Sodium chloride
<% } %> <% if (tris_base) { %>
<%= tris_base.decimal_rtrim(NUMERICIAL_PRECISION) %> g of Tris base
<% } %>
2

Check pH

<% if (media_name === FaSSGF || media_name == DOGFASSGF) { %>

Adjust the pH to <%= ph %> with HCl.

<% } else { %>

Adjust the pH to <%= ph.toFixed(1) %> with <% if(ph != 1.5 && ph != 1.6) { %>Sodium hydroxide 1N or<% } %> Hydrochloric acid 1N.

<% } %>

Make up to volume (<%= amount.decimal_rtrim(NUMERICIAL_PRECISION) %> L) with purified water at room temperature.

3

Add powder

Add <%= sif.decimal_rtrim(NUMERICIAL_PRECISION) %> g of <%= product_name %> to about <%= buffer %> L <% if (media_name === FaSSGF) { %> HCl/NaCl solution. <% } else { %> of buffer.<% } %>

<% if (media_name === FaSSGF) { %>

Make up to volume (<%= amount.decimal_rtrim(NUMERICIAL_PRECISION) %> L) with purified water at room temperature.

<% } else { %>

Stir until powder is completely dissolved.

Make up to volume (<%= amount.decimal_rtrim(NUMERICIAL_PRECISION) %> L) with buffer at room temperature.

<% } %>
4

Ready to use

<% if (media_name === 'FaSSIF') { %>

Let stand for 2 hours. It will become slightly opalescent. Your FaSSIF is now ready to use.

<% } else if (media_name === 'FeSSIF' ) { %>

FeSSIF will have a clear appearance and is now ready to use.

<% } else if (media_name === 'FaSSGF' ) { %>

Your FaSSGF will have a clear appearance and is ready to use.

<% } else if (media_name === 'FaSSIF-V2' ) { %>

Let stand for 1 hour. Your FaSSIF-V2 is now ready to use.

<% } else if (media_name === 'FeSSIF-V2' ) { %>

Let stand for 1 hour. FeSSIF-V2 will have a clear appearance and is now ready to use.

<% } else if (media_name === 'DOGFASSGF') { %>

Your Dog FaSSGF will have a clear appearance and is ready to use.

<% } else if (media_name === 'DOGFASSIF') { %>

Let stand for 1 hour. Your Dog FaSSIF is now ready to use.

<% } else if (media_name === 'FaSSCoF') { %>

Let stand for 1 hour. Your FaSSCoF is now ready to use.

<% } else if (media_name === 'FeSSCoF') { %>

Let stand for 1 hour. Your FeSSCoF is now ready to use.

<% } %>
ADDITIONAL TIPS
<% if (media_name === 'FaSSCoF' || media_name === 'FeSSCoF') { %> Do not freeze or refrigerate <%= media_name %>. We recommend using it at room temperature or 37°C. It can be used for up to 24 hours at these temperatures <% } else if (media_name === 'DOGFASSIF' ) { %> Do not freeze or refrigerate Dog FaSSIF. We recommend using it at room temperature or 37°C. It can be used for up to 48 hours at these temperatures <% } else { %> Do not freeze or refrigerate <%= media_name %>. We recommend using it at room temperature or 37°C. It can be used for up to 48 hours at these temperatures <% } %>
It's important to use clean glassware for making <%= media_name %> in order to prevent microbial growth
For the buffer preparation, we advise using deionised water and also degassing it
Once opened, store the bottle of <%= product_name %> in the refrigerator (2-8C) with the cap tightly closed
Keep <%= media_name %> out of direct sunlight and preferably in the dark
It's possible to make a large volume of blank buffer which can be stored for up to 3 months. We suggest sterilizing this buffer and keeping it refrigerated
<% if (media_name === 'FaSSGF') { %>
If required, 0.1 mg/ml of pepsin can be added to FaSSGF after preparation
<% } %> <% if (media_name === 'FaSSIF') { %>
If the opalescence of FaSSIF interferes with your UV assay, we recommend letting the medium stand for 4 hours before use (rather than the usual 2 hours)
<% } %> <% if (media_name === 'FeSSCoF' || media_name === 'FaSSCoF') { %>
The <%= media_name %> powder is very hygroscopic so it's important to replace the bottle cap immediately after use and keep it tightly closed
<% } %>