Analysing your drug in Biorelevant Media is quite simple; it is just like analysing drugs in other test solutions.

After taking a dissolution sample containing either your Test Formulation or Reference, the first step is to immediately separate undissolved drug from drug dissolved in the Biorelevant Media. Filtration is the most common and preferred separation technique to remove undissolved drug. Centrifugation may also be used to spin down undissolved drug but is not routinely recommended for certain fed state media (such as FEDGAS) which contain larger colloidal particles.

After drug separation, the medium (containing dissolved drug) is best analysed by HPLC. HPLC avoids potential interference of the media and can identify possible drug instability.

For more biorelevant dissolution analytical tips, download our Dissolution Guides here.