How to analyse your drug in biorelevant media

Analysis of your drug in biorelevant media is straightforward; the method required should be similar to those used for analysing drugs in other test solutions.

The first step is to separate undissolved drug from drug dissolved in the biorelevant media. Filtration is the most common separation technique to remove undissolved drug. Centrifugation may also be used to spin down undissolved drug but may not be appropriate for some fed state media. If you’d like to discuss this in more detail with our Technical Support team, get in touch with them here.

After drug separation, the medium (containing dissolved drug) is best analysed by HPLC. HPLC avoids potential interference of the media and can identify possible drug instability.