Dilution of secondary standard (SS)

The dilution of standard containing biorelevant medium is slightly different from the biorelevant dissolution sample:  a working standard (WS) is typically prepared by combining the SSS, diluent and, biorelevant medium together. To prepare a 1:10 dilution ratio, combine 0.100mL of SS, 0.800mL of diluent and, 0.100mL of biorelevant medium, so the final volume will be 1.000mL. The total mL of diluent in 1.000mL will be 0.900mL as the SS is also included in the dilution.

Dilution of biorelevant dissolution sample

The dilution of the biorelevant dissolution sample is straightforward: for a 1:10 dilution accurately dispense 0.100mL of biorelevant dissolution sample solution and add 0.900mL of diluent, yielding a final volume of 1.000mL.

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