Biorelevant has recently introduced a Buffer Concentrate to be used with FaSSIF-V2 Powder. To compare HPLC profiles of FaSSIF-V2 made using this Buffer Concentrate and FaSSIF-V2 made using buffer from scratch, the following HPLC in-house method has been used throughout the different experiments.

Example of blank FasSIF-V2 media

The chromatogram of FaSSIF-V2 made using Buffer Concentrate (above left) shows a much cleaner HPLC profile avoiding the extra peak which can be observed when using the Maleic acid buffer made from scratch (above right).

HPLC Chromatograms of three drugs in improved FaSSIF-V2 made using Buffer Concentrate (in green) compared with FaSSIF-V2 made using buffer from scratch (in orange) 

A peak attributable to the Maleic acid is present in FaSSIF-V2 made using buffer from scratch but not present in the FaSSIF-V2 made using Buffer Concentrate.

Typical chromatographic parameters, showing the similarities of both media, are given in the table below. The samples have been diluted with the diluent 50% MeOH : 50% Phosphate Buffer pH 2.0 20mM at a 1:4 v/v ratio.