Benefits

Biorelevant is delighted to introduce our new FeSSIF Buffer Concentrate to make your Biorelevant Media preparation even quicker, easier and more consistent. Each bottle will make up to 3litres of FeSSIF buffer. Simply dilute the concentrate to the amount of buffer you require and combine with FaSSIF/FeSSIF/FaSSGF powder (sold separately) to produce excellent results every time.

*Saves Time*

Make virtually any volume of FeSSIF you require in less than 5 minutes:

*Reproducible Results*

Each bottle comes with a Certificate of Analysis to guarantee quality. This graph shows the reproducible pH measurements of FeSSIF produced with the Buffer Concentrate:

• 1118A: pH average 5.00, standard deviation 0.02, 30 samples measured
• 0219A: pH average 4.99, standard deviation 0.02, 8 samples measured

*Control*

Convenient packaging gives you precise control over your media preparation. Either small or large volumes can be prepared with ease and minimal waste.

1L of FeSSIF made with Buffer Concentrate

Equipment

• Calibrated balance
• Suitable container
• Stirrer plate and PTFE magnet
• Weighing boat

Method

• Weigh 81.41g (or 70.00mL) of buffer concentrate into a suitable container
• Weigh 919.4g of purified water into container to produce buffer
• Weigh 11.20g of FaSSIF/FeSSIF/FaSSGF powder into weighing boat and then add to container. Rinse weighing boat with buffer to ensure all powder has been transferred
• Place container on stirrer plate with PTFE magnet. Leave to stir until dissolved
• FeSSIF medium will have a clear appearance and is ready to use

Usage

• Use FeSSIF within 48 hours