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ALL Background Analysis Dissolution Filters Biorelevant media NEW!
What is FaSSIF?
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FaSSIF (Fasted State Simulated Intestinal Fluid) is a very useful drug development tool. Tests in this dissolution medium can reveal …
What is FeSSIF?
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FeSSIF (Fed State Simulated Intestinal Fluid) is a very useful drug development tool. Tests in this dissolution medium can help …
What is FaSSGF?
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FaSSGF (Fasted State Simulated Gastric Fluid) is a very useful drug development tool. Tests in this dissolution medium can help …
What is FEDGAS?
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FEDGAS (Fed State Simulated Gastric Fluid AKA FeSSGF) is a very useful drug development tool. Tests in these dissolution media …
FeSSGF vs FEDGAS: a comparison
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In vitro dissolution testing of drugs in media that simulate in vivo gastric fluids after a meal can be very …
How to analyse your drug in Biorelevant Media
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After drug separation, the medium (containing dissolved drug) is best analysed by HPLC. HPLC avoids potential interference of the media and can identify possible drug instability
How biorelevant media can help develop new drugs (NCEs)
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Whether you're a chemist, biologist or pharmacist involved in the development a new oral drug (NCE: New Chemical Entity), biorelevant …
An improved buffer concentrate for preparation of FaSSIF-V2
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An improved buffer concentrate for preparation of FaSSIF-V2 has been developed to overcome the limitations of the previous maleic acid buffer system.
HPLC Parameters using FaSSIF-V2
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Comparing HPLC profiles of FaSSIF-V2 made using Buffer Concentrate versus FaSSIF-V2 made using buffer from scratch
Dissolution profiles in FaSSIF-V2
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Ibuprofen, Carbamazepine and Diclofenac dissolution profiles in FaSSIF-V2
An improved Buffer Concentrate for preparation of FeSSIF-V2
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An improved Buffer Concentrate for preparation of FeSSIF-V2 has been developed to overcome the limitations of the previous maleic acid buffer system.
HPLC Parameters using FeSSIF-V2
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To compare the HPLC profiles of FeSSIF-V2 prepared using a) FeSSIF-V2 Powder with FeSSIF-V2 Buffer Concentrate and b) FeSSIF-V2 Powder with buffer made from scratch, the following HPLC in-house method has been used throughout the different experiments.
Dissolution profiles in FeSSIF-V2
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Dissolution runs, using USP Dissolution Apparatus 2, were carried out at 37°C with 900mL of the FeSSIF-V2 media.
Composition and properties of FaSSIF, FeSSIF and FaSSGF Media prepared from 3F Powder and Buffer Concentrates
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FaSSIF, FeSSIF and FaSSGF dissolution media are prepared by diluting FaSSIF, FeSSIF, FaSSGF Buffer Concentrates with water and adding the required amount of 3F Powder®.
pH reproducibility of FaSSIF, FeSSIF and FaSSGF prepared from 3F Powder® and corresponding Buffer Concentrates
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Variable pH of a solubility or dissolution medium is one of the most common and significant reasons for irreproducible results which affects decision making and interpretation.
What is a diluent?
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A diluent is a solvent which is added to standard solutions of a drug substance...
Why include Biorelevant Medium in working standard solutions (WSS)
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It is important to include Biorelevant Medium when diluting secondary standard solutions (SSS) as it keeps the matrix...
Why Biorelevant Dissolution samples should be diluted for HPLC analysis
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Without dilution, drugs which are poorly water soluble may precipitate from biorelevant medium at room temperature after sampling.
How to examine compatibility and dilution ratio of diluent with Biorelevant Media
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Before HPLC analysis of any biorelevant dissolution sample containing drug, it is important to have identified a compatible diluent for the biorelevant media that will be used.
How to calculate the theoretical maximum drug concentration for Primary Standard Solution (PSS)
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Before making the PSS, calculate the theoretical maximum drug concentration required for analysis...
How to dilute and prepare standard solutions
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There are three types of standard solutions that need to be prepared when analysing dissolution samples...
Which dilution ratio to select for HPLC analysis
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A suitable diluent ratio should not only keep the sample chemically and physically stable but should also allow the drug to be detected accurately and precisely by HPLC.
How to prepare the working drug solution containing dosage form (e.g. drug and excipients) with biorelevant medium to check interference
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Three steps are required to prepare the working drug solution containing dosage form...
Which solutions are required to check for interference
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The following solutions should be tested to check for interference...
How to dilute your secondary standard solution (SSS) and dissolution samples with 1:10 dilution ratio whilst keeping the same matrix
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The dilution of standard solutions containing biorelevant medium is slightly different from the biorelevant dissolution sample...
Selecting the drug concentration range of linearity working standard solutions
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The linearity of an analytical method enables (over the range of dissolution concentration) generation of test results which are directly proportional to the concentration of drug substance in the dissolution sample.
NEW!
Importance of filtration in dissolution sample preparation for HPLC analysis
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Filtration is a crucial step in preparing a dissolution sample. It involves passing the sample through a filter to separate solid particles or undissolved materials...